Dr. Brijesh N

Assistant professor and Head of the Department

Qualification: M.Sc., Ph.D

Courses taught: MSc Biotechnology, BSc Biotechnology

Area of expertise: Glycobiology including Proteomics, Glycomicis and lipidomics (Isolation, purification and characterization of biomolecules) along with cells based bioassays

Contact details: brijesha.n@sdmmmkmysore.in

National/International Symposia Presentation and Participation

  1. 103rd INDIAN SCIENCE CONGRESS -2016, Manasagangotri, University of Mysore, Mysore-570006 January 3-7, 2016.
  2. Interaction of peptides from milk fat globule membrane proteins with oxidants offers cellular protection. Drug discovery and development, Mysore. December 13-15, 2015.
  3. Glycomics of fat globule membrane from Buffalo milk and colostrum - Diversity in sugar epitopes of glycoconjugates. Chemistry and Chemical Biology (India), Manasagangothri, Mysore. May, 26-05, 2015.
  4. Glycomics of fat globule membrane from Buffalo milk and Colostrum. DOS in Biotechnology, University of Mysore, Mysuru, Karnataka and National academy of Biological sciences, Chennai, Tamilnadu. 21-22 August, 2015 - Fat globule membrane proteins from Buffalo (Bubalus bubalis) colostrums and their functional significance 27th International carbohydrate symposium, Indian Institute of Science, Bangalore, January 12-17, 2014.

Workshops Attended

  1. "Intellectual Property Training and Awareness" Vijnanabhavan, Manasagangothri, Mysore- 570006.
  2. "Computational Methods in Small Molecule and Bio-Pharmaceutical Drug Discovery". Institute Of Excellence, University Of Mysore, Mysuru-570006.

Research Details

  1. Isolation of Fat globule membrane glycoconjugates from milk and colostrum. * SDS-PAGE analysis for major proteins identification.
  2. In vitro gastrointestinal simulation digestion using proteolytic enzymes and separation of small peptides.
  3. LC/ESI-MS/MS based fragmentation techniques.
  4. Protein - protein interaction studies by in silico methods.
  5. Glycans separation from milk and colostrum fat globules using Trypsin and PNGase F enzymes.
  6. Characterization of glycans by MALDI-TOF MS/MS.
  7. Functional properties of peptides - antioxidant studies.
  8. Antimicrobial activity of glycans against clinically relevant pathogens.

Title of the Paper publications:

  1. Brijesha N and Aparna H S. Comprehensive characterization of bioactive peptides from Buffalo (Bubalus bubalis) colostrums and milk fat globule membrane proteins. Food Research International, 97: 95-103, 2017. (IF- 3.790, Citation - 6)
  2. Brijesha N, Nishimura S-I. and Aparna H S. A comparative glycomics of fat globule membrane glycoconjugates from Buffalo (Bubalus bubalis) milk & colostrum. Journal of Agricultural and Food Chemistry, 65: 1496-1506, 2017. (IF- 4.192, Citation - 4)
  3. Brijesha N and Aparna H S. Buffalo (Bubalus bubalis) colostrum and milk fat globule membrane fractions are potent antioxidants. Journal of Applied Biology & Biotechnology, 5: 89-93, 2017. (ISSN No. 2455-7005, Citation - 2).
  4. Arpitha Ashok, Brijesha N and Aparna H S. Discovery, synthesis and in vitro evaluation of novel bioactive peptide with ACE and DPPIV inhibitory activity. European journal of medicinal chemistry, 180: 99-110, 2019. (IF- 5.572, Citation - 6)